Sequencing Graph

In this experiment we learned how to sequence DNA using the Sanger method.  In this method you write the letters down in the order they come up in the sequence.  Then you set them up in groups of threes and select the protein that corresponds with the group.  The first patient Abby had a point mutation, which means just one letter is different.  The next patient Bob had one thing wrong, he had a trancation mutation.  This is when it stops and can't finish the sequence.  The next patient Carol had 18 letters wrong.  This was because she had a frameshift mutation.  This is when one sequence loses or gains a letter and then makes the rest of the sequence off.  That is how you read a sequence and the three things that can go wrong.