Sequencing Graph

In this experiment we learned how to sequence DNA using the Sanger method.  In this method you write the letters down in the order they come up in the sequence.  Then you set them up in groups of threes and select the protein that corresponds with the group.  The first patient Abby had a point mutation, which means just one letter is different.  The next patient Bob had one thing wrong, he had a trancation mutation.  This is when it stops and can't finish the sequence.  The next patient Carol had 18 letters wrong.  This was because she had a frameshift mutation.  This is when one sequence loses or gains a letter and then makes the rest of the sequence off.  That is how you read a sequence and the three things that can go wrong.

In Sickness and in health

In Sickness and in Health

          The picture above is a model of Greg and Olga's pedigree's.  The first problem was myotonic dystrophy.  This disease is autosomal dominant.  An autosomal dominant disease can not skip a generation so they don't have to worry about passing it on to  their children.  The next disease they approached was factor VIII deficiency.  This disease is sex linked.  In a sex linked disease it can only be carried by the male because these diseases are on the X chromosome.  This means that the disease will only affect a boy if it is passed down.  The las thing they talked about was cystic fibrosis.  This is an autosomal recessive disease.  This means that it can skip generations.  Some people can be carriers of a disease like this.  It is a slight chance that their child can get it though.  Some races have more people that carry it over others too.  Their baby ended up having a 1 in 16, 560.  In the end the kid has a very slim chance of getting any diseases even though there are diseases in the family.